Titration of non-occluded baculovirus using a cell viability assay.

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line.

A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter. When used in the titration assay, the Sf-9ET cells turn green when they are infected with baculov...

Automated baculovirus titration assay based on viable cell growth monitoring using a colorimetric indicator.

Evaluation of MTT and Trypan Blue assays for radiation-induced cell viability test in HepG2 cells

Background:  Cell viability is an important factor in radiation therapy and thus is a method to quantify the effect of the therapy. Materials and Methods: The viability of human hepatoma (HepG2) cells exposed to radiation was evaluated by both the MTT and Trypan blue assays. The cells were seeded on 96 well-plates at a density of 1 x 104 cells/well, incubated overnight, and irradiated with...

A Quick and Efficient Method to Quantify Baculo Virus by Quantitative Real Time PCR

Recombinant protein expression using the Baculovirus Expression System is widely used for the production of therapeutics, diagnostics, and reagents. The current “gold standard” for Baculovirus titration, the plaque assay, is labor intensive and time consuming. The realtime PCR assay has the advantage of speed and accuracy. We determined Baculovirus tire by a novel quantitative PCR (qPCR) method...

In-vitro study of Radioprotection Effects of Cerium Oxide Nanoparticles in Exposure to MRC-5 Fibroblastic Cell lines with 6MV Photon Beams Using MTT Assay

Introduction: Recently Cerium oxide nanoparticles (CONPs) are being checked as interventional treatments in biological systems. The scavenging of free radicals by nanoparticles performance is the inhibition of Reactive Oxygen Species (ROS). Ionizing radiations can prevent the proliferation and differentiation of cells and even cause apoptosis. CONPs, as radioprotection, can pro...